Mutation opgB202::Tn10
- Name: opgB202::Tn10
- Type: Tn
- Mutation of Gene: opgB
- Approx. Map Location: 99.04
- Synonyms
Designation Where Used mdoB202::Tn10 zii-202 zij-202 - Properties
Property Symbol tetracycline resistant - Comments
- cotransduces 34% with serB and 13% with thr and ca. 50% with hsd. Shown by PCR analysis (Nichols et al.) to be within opgB at nucleotide 4,595,002 (99.0 min.).
- References
- Wertman, K.F., A.R. Wyman, D. Botstein 1986. Host/vector interactions which affect the viability of recombinant phage lambda clones. Gene 49:253-262
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
- 10 Strains Carrying This Mutation
Name Mutations Genotype CAG18430 3 F-, λ-, rph-1, opgB202::Tn10 DB1316 5 F-, λ-, recD1014(Nuc-), mcrB1, hsdR2, opgB202::Tn10 DB1318 5 F-, λ-, recA938::Tn9-200, recD1014(Nuc-), hsdR2, opgB202::Tn10 DB1317 6 F-, λ-, tyrT58(AS), trp-89::Tn5, recD1014(Nuc-), hsdR2, opgB202::Tn10 SK596 6 Hfr(PO1), λ-, e14-, relA1, spoT1, thiE1, opgB202::Tn10 DB1319 7 F-, λ-, tyrT58(AS), trp-89::Tn5, recA938::Tn9-200, recD1014(Nuc-), hsdR2, opgB202::Tn10 MOB145 7 Hfr(PO1), gal-55, LAM?, relA1, spoT1, thiE1, hsdR4, opgB202::Tn10 SK472 7 Hfr(PO1), λ-, e14-, relA1, spoT1, thiE1, opgB202::Tn10, serB22 W3110trpC720(del) 7 F-, λ-, ΔtrpC720, IN(rrnD-rrnE)1, rph-1, tnaA5, hsdR2, opgB202::Tn10 LCK8 8 F-, lacY1 or Δ(cod-lacI)6, glnX44(AS), galK2(Oc), galT22, λ-, metB1, hsdR2, opgB202::Tn10