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Mutation mutH472::mini-Tn10

• Name: mutH472::mini-Tn10
• Type: Tn
• Mutation of Gene: mutH
• Approx. Map Location: 63.97
• Synonyms

  Designation	Where Used
  fpg-
  mutM472(p)::mini-Tn10	Michaels et al.

  
  
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • This insertion is not in mutM and the mutator has G to A specificity rather than G to T. PCR confirms that it is in the mutH gene, at bp 628 of the 690 bp CDS (M.Volkert, pers.commun.2000)
• References
  • Michaels, M.L., L. Pham, C. Cruz, J.H. Miller 1991. MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19:3629-3632
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  MC301	8	F-, ara-600, Δ(gpt-lac)5, λ-, gyrA284(NalR), relA1?, mutH472::mini-Tn10, spoT1?, thiE1?