E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Mutation DE(ompT-nfrA)885

• Name: DE(ompT-nfrA)885
• Type: Deletion
• Approx. Map Location: 12.59
• Synonyms

  Designation	Where Used
  ompT504(del)

  
  
• Properties

  Property	Symbol
  deletion	del

  
  
• Comments
  • This deletions actually spans from distal region of the DLP12 prophage to an Rhs element just downstream of nfrA in the E. coli B genome. Actual genes deleted are appY, ompT, envY, ybcH, and nfrA.
• References
  • Studier, FW, P Daegelen, RE Lenski, S Maslov, JF Kim 2009. Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes. J. Mol. Biol. 394(4):653-80.
• 6 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BA-002	3	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, ?ptsI845::FRT, [mal+]K-12(λS), hsdS10
  WA834	6	F-, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, Δ46, met-100, [mal+]K-12(λS), hsdS10
  BL21(DE3)	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10
  BL21(DE3)/pCP20	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pCP20
  BL21(DE3)/pKD46	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pKD46
  BL21(DE3)/pLysS	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pLysS