E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Mutation priA1::KanR

• Name: priA1::KanR
• Type: Tn
• Mutation of Gene: priA
• Approx. Map Location: 88.86
• Comments
• References
  • Nurse, P., R.J. DiGate, K.H. Zavitz, K.J. Marians 1990. Molecular cloning and DNA sequence analysis of Escherichia coli priA, the gene encoding the primosomal protein replication factor Y. Proc.Natl.Acad.Sci.USA 87:4615-4619
  • Nurse, P., K.H. Zavitz, K.J. Marians 1991. Inactivation of the Escherichia coli PriA DNA replication protein induces the SOS response. J.Bacteriol. 173:6686-6693
  • Xu, L., K.J. Marians 2000. Purification and characterization of DnaC810, a primosomal protein capable of bypassing PriA function. J.Biol.Chem. 275:8196-8205
• 5 Strains Carrying This Mutation

  Name	Mutations	Genotype
  PN105	5	F-, leu::Tn10, sulA366, his-, rpsL-(strR), priA1::KanR
  PN102	11	Hfr(PO2A), garB10, fhuA22, phoA4(Am), ompF627(T2R), serU132(AS), fadL701(T2R), relA1, pitA10, spoT1, priA1::KanR, mcrB1
  PN103	12	Hfr(PO2A), garB10, fhuA22, phoA4(Am), λ-, ompF627(T2R), serU132(AS), fadL701(T2R), relA1, pitA10, priA1::KanR, mcrB1, creC510
  PN104	15	Hfr(PO2A), garB10, fhuA22, lacZ8(Am), phoA4(Am), λ-, ompF627(T2R), fadL701(T2R), relA1, pitA10, dinD1::Mud(Ap,lac), spoT1, priA1::KanR, rrnB-2, mcrB1, creC510
  PN101	23	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, sbcC201, tsx-33, glnX44(AS), galK2(Oc), λ-, Rac-0, sbcB15, hisG4(Oc), rfbC1, recB21, recC22, rpsL31(strR), kdgK51, xylA5, mtl-1, priA1::KanR, argE3(Oc), thiE1