E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation phi80dlacI+

• Name: phi80dlacI+
• Type: Insertion
• Approx. Map Location: 28.21
• Comments
  • The ?80dlacI⁺ was constructed by integrating the ?80dlac⁺ prophage and then selecting for tonB mutants that also deleted the lacZYA genes leaving the lacZ promoter.
• References
  • Ippen, K, JH Miller, J Scaife, J Beckwith 1968. New controlling element in the Lac operon of E. coli. Nature 217(5131):825-7.
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  KL340	6	F', Δ(gpt-lac)5, φ80dlacI+, Δ(att80-tonB)886, rpsE2129(SpcR), xyl-7, mtlA2, F42-17