E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation LAMDE3

• Name: LAMDE3
• Type: Insertion
• Approx. Map Location: 17.38
• Comments
  • Lambda DE3 has the phage T7 RNA polymerase under the control of the lacUV5 promoter inserted into the BamHI site of the Lambda cloning vector D69.
• References
• 7 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BA-002	3	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, ?ptsI845::FRT, [mal+]K-12(λS), hsdS10
  HMS174(DE3)	6	F-, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], recA1, IN(rrnD-rrnE)1, rph-1?, rpoB331(rifR), hsdR19
  HMS174(DE3)/pLysS	6	F-, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], recA1, IN(rrnD-rrnE)1, rph-1?, rpoB331(rifR), hsdR19, pLysS
  BL21(DE3)	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10
  BL21(DE3)/pCP20	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pCP20
  BL21(DE3)/pKD46	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pKD46
  BL21(DE3)/pLysS	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pLysS