E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation DE(ruvB-ruvC)798::(apra)

• Name: DE(ruvB-ruvC)798::(apra)
• Type: Deletion
• Approx. Map Location: 41.86
• Properties

  Property	Symbol
  Apramycin resistant	apra

  
  
• Comments
  • The deletion starts 39bp into ruvC and extends to 39bp from the end of ruvB. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and apramycin cassette in lowercase: TCGCGCGTG/tcatgtgca..........tctgggcgg/TGGAATCAC.
• References
  • Rudolph, CJ, AA Mahdi, AL Upton, RG Lloyd 2010. RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli. Genetics 186(2):473-92.
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  N7105	3	F-, λ-, Δ(ruvB-ruvC)768(::apra), rph-1