Mutation lacZ4821

Name: lacZ4821
Type: Compound Allele
Mutation of Gene: lacZ
Approx. Map Location: 7.81
Synonyms
Designation Where Used
lacZ(QP5) Seier et al., 2011
Comments
- is a compound allele replacing TTTG-C--CTGGT-TTCCGGCACCAGAA with TTTGCCAGCTTCTCTTCCGGCACCGGAA starting at base 181 in the lacZ sequence. This results in a frameshift mutation and the formation of a quasipalendrome.
References
- Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.
2 Strains Carrying This Mutation
Name Mutations Genotype
STL14553 4 F-, lacZ4821, mhpC281::Tn10, λ^-, rph-1
STL14778 5 F-, lacZ4821, mhpC281::Tn10, λ^-, ΔmutS738::kan, rph-1

Name	Mutations	Genotype
STL14553	4	F-, lacZ4821, mhpC281::Tn10, λ^-, rph-1
STL14778	5	F-, lacZ4821, mhpC281::Tn10, λ^-, ΔmutS738::kan, rph-1

E. coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center