E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation crcA280::Tn10

• Name: crcA280::Tn10
• Type: Tn
• Mutation of Gene: crcA
• Approx. Map Location: 14.14
• Synonyms

  Designation	Where Used
  ybeG::Tn10
  zbe-280::Tn10

  
  
• Properties

  Property	Symbol
  tetracycline resistant

  
  
• Comments
  • originally in SK2257, cotransduces 76% with leuS and also cotransduces with rna. Shown by PCR (Nichols et al.) to be at nucleotide 655,952 (14.139 min.).
• References
  • Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.
• 3 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CAG12077	3	F-, crcA280::Tn10, λ-, rph-1
  CAG5053	4	Hfr(PO43), crcA280::Tn10, λ-, relA1?, spoT1?
  SK2257	4	F-, crcA280::Tn10, thyA6, rpsL120(strR), deoC1?