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CGSC, The Coli Genetic Stock Center

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Mutation leuB261(del)::lacZp-lacI-mCherry,cat

• Name: leuB261(del)::lacZp-lacI-mCherry,cat
• Type: Insertion
• Mutation of Gene: leuB
• Approx. Map Location: 1.74
• Properties

  Property	Symbol
  chloramphenicol resistant
  deletion	del

  
  
• Comments
  • This allele has a lacZp promoter driving expression of a lacI-mCherry fusion protein. This is followed by a chloramphenicol resistance cassette.
• References
  • Reyes-Lamothe, R, C Possoz, O Danilova, DJ Sherratt 2008. Independent positioning and action of Escherichia coli replisomes in live cells. Cell 133(1):90-102.
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  RRL150	21	F-, thr-1, araC14, ΔleuB261::lacZp-lacI- mCherry,cat, Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), ΔgalK539::lacZp-tetR-mCerulean,kan, λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1