E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Mutation galK539(del)::lacZp-tetR-mCerulean,kan

• Name: galK539(del)::lacZp-tetR-mCerulean,kan
• Type: Insertion
• Mutation of Gene: galK
• Approx. Map Location: 16.99
• Properties

  Property	Symbol
  deletion	del
  kanamycin resistant

  
  
• Comments
  • This allele has a lacZp promoter driving expression of a tetR-mCerulean fusion protein. This is followed by a kanamycin resistance cassette.
• References
  • Reyes-Lamothe, R, C Possoz, O Danilova, DJ Sherratt 2008. Independent positioning and action of Escherichia coli replisomes in live cells. Cell 133(1):90-102.
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  RRL150	21	F-, thr-1, araC14, ΔleuB261::lacZp-lacI- mCherry,cat, Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), ΔgalK539::lacZp-tetR-mCerulean,kan, λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1