E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation zie-3627::lacZo(240X)::kan

• Name: zie-3627::lacZo(240X)::kan
• Type: Insertion
• Approx. Map Location: 84.27
• Properties

  Property	Symbol
  kanamycin resistant

  
  
• Comments
  • This allele has a kan cassette between two arrays of 120 tandem repeats of the lacZ operator site, all of which is inserted at the attTn7 locus.
• References
  • Lau, IF, SR Filipe, B Søballe, OA Økstad, FX Barre, DJ Sherratt 2003. Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol. Microbiol. 49(3):731-43.
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  IL01	22	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, zie-3627::lacZo(240X)::kan, argE3(Oc), thiE1
  IL06	23	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, zdf-3630::tetAo (240X)::aacC1, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, zie-3627::lacZo(240X)::kan, argE3(Oc), thiE1