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CGSC, The Coli Genetic Stock Center

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Mutation thyA762(del)::kan

• Name: thyA762(del)::kan
• Type: Insertion
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Properties

  Property	Symbol
  deletion	del
  kanamycin resistant

  
  
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
  • The primers used to make this knockout were GGCGTCGGCTCTGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTATTCCGGGGATCCGTCGACC and TCTGGGCATATCGTCGCAGCCCACAGCAACACGTTTCCTGAGGAACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• References
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11