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CGSC, The Coli Genetic Stock Center

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Mutation argU110(ts)

• Name: argU110(ts)
• Type: Unknown
• Mutation of Gene: argU
• Approx. Map Location: 12.14
• Synonyms

  Designation	Where Used
  argU10
  dnaY10

  
  
• Properties

  Property	Symbol
  Temperature Sensitive	ts

  
  
• Comments
  • Pleiotropic properties of argU110(ts) mutant result fr. reduced intracell/ levels of this minor tRNA(Arg)--ca. 1/10 WT level, further reduced at 43C. reduction prob.due to defective interx.w/ Tu. ArgS
• References
  • Sakamoto, K., S. Ishimaru, T. Kobayashi, J.R. Walker, S. Yokoyama 2004. The Escherichia coli argU10(Ts) phenotype is caused by a reduction in the cellular level of the argU tRNA for the rare codons AGA and AGG. J.Bacteriol. 186:5899-5905
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  GM10	17	F-, thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, argU110(ts), glnX44(AS), gal-6, λ-, hisG1, rfbC1, galP63, xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1