Mutation efeU'-762(del)::kan
- Name: efeU'-762(del)::kan
- Type: Insertion
- Mutation of Gene: efeU'
- Approx. Map Location: 23.29
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- efeU' is a pseudogene due to frameshift mutation in K-12. In efeUOB strains, it encodes membrane-spanning subunit of EfeUOB, Fur-dependent inducible low-pH ferrous iron transporter EfeUOB.
- The primers used to make this knockout were TTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGATTCCGGGGATCCGTCGACC and CGCAGCGGCAAGCAACACGCCAATCCACATCACACAATCCATCGGCCTCGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
- Grosse, C., J. Scherer, D. Koch, M. Otto, N. Taudte, G. Grass 2006. A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. Mol.Microbiol. 62:120-131
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5141-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔefeU'-762::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514