Mutation sad-746(del)::kan
- Name: sad-746(del)::kan
- Type: Insertion
- Mutation of Gene: sad
- Approx. Map Location: 34.73
- Synonyms
Designation Where Used yneI746(del)::kan original temporary nomenclature - Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACATTCCGGGGATCCGTCGACC and AATATTATTCATTTTAAAGCAAGAGTAAATCTGCGTATCTTCATACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
- Fuhrer, T., L. Chen, U. Sauer, D. Vitkup 2007. Computational Prediction and Experimental Verification of the Gene Encoding the NAD+/NADP+-Dependent Succinate Semialdehyde Dehydrogenase in Escherichia coli. J.Bacteriol. 189:8073-8078
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5247-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δsad-746::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514