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CGSC, The Coli Genetic Stock Center

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Mutation spy-741(del)::kan

• Name: spy-741(del)::kan
• Type: Insertion
• Mutation of Gene: spy
• Approx. Map Location: 39.30
• Properties

  Property	Symbol
  deletion	del
  kanamycin resistant

  
  
• Comments
  • The primers used to make this knockout were CGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATATTCCGGGGATCCGTCGACC and CTACTTAAGAAAGCCGTAATAAATAACTGAAAGGAAGGATATAGAATATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• References
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  JW1732-1	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δspy-741::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514