Mutation smf-724(del)::kan
- Name: smf-724(del)::kan
- Type: Insertion
- Mutation of Gene: smf
- Approx. Map Location: 73.94
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GACCAATCGCAAAGATTGCTAAGGCTGCTTATGGCAGGGAGATAAGGATGATTCCGGGGATCCGTCGACC and GCAACTCAGCTTCTGTGTGAATATAGGTTTCAAACAAATACATTAGTACGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5708-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δsmf-724::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
