Mutation ompT774(del)::kan

Name: ompT774(del)::kan
Type: Insertion
Mutation of Gene: ompT
Approx. Map Location: 12.59
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were TCACCTCGGGGAAATTTTAGTTGGCGTTCTTAAAATGTGTACTTAAGACCATTCCGGGGATCCGTCGACC and TAAAAAATACATATTCAATCATTAAAACGATTGAATGGAGAACTTTTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
2 Strains Carrying This Mutation
Name Mutations Genotype
WTZ16002 5 F-, ΔlacY884::FRT, ΔompT774::kan, λ^-, IN(rrnD-rrnE)1, rph-1
JW0554-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔompT774::kan, λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514

Name	Mutations	Genotype
WTZ16002	5	F-, ΔlacY884::FRT, ΔompT774::kan, λ^-, IN(rrnD-rrnE)1, rph-1
JW0554-1	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔompT774::kan, λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514

Property	Symbol
deletion	del
kanamycin resistant

E. coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center