Mutation ldcA782(del)::kan

Name: ldcA782(del)::kan
Type: Insertion
Mutation of Gene: ldcA
Approx. Map Location: 26.76
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- Due to an irregularity in the nomenclature the mnemonic for this gene should be L,D-carboxypeptidase A rather than lysine decarboxylase
- The primers used to make this knockout were GTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGATTCCGGGGATCCGTCGACC and AAGCGGCATGATATACATTTCGTTTCTGTTGTCAGCAAGGAATTGCCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
1 Strains Carrying This Mutation
Name Mutations Genotype
JW1181-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔldcA782::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514

Property	Symbol
deletion	del
kanamycin resistant

E. coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center