E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Mutation fre-784(del)::kan

• Name: fre-784(del)::kan
• Type: Insertion
• Mutation of Gene: fre
• Approx. Map Location: 86.74
• Properties

  Property	Symbol
  deletion	del
  kanamycin resistant

  
  
• Comments
  • The primers used to make this knockout were CGCCTGTCAGGGGCGGGTTTTTTTATATCTCAGATAAATGCAAACGCATCATTCCGGGGATCCGTCGACC and GATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• References
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
• 1 Strains Carrying This Mutation

  Name	Mutations	Genotype
  JW3820-1	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δfre-784::kan, Δ(rhaD-rhaB)568, hsdR514