E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation DE46

• Name: DE46
• Type: Deletion
• Approx. Map Location: 46.69
• Comments
  • : cured of defective P2-like prophage found in wildtype E.coli B.
• References
  • Studier, FW, P Daegelen, RE Lenski, S Maslov, JF Kim 2009. Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes. J. Mol. Biol. 394(4):653-80.
• 8 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BA-002	3	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, ?ptsI845::FRT, [mal+]K-12(λS), hsdS10
  WA707	4	F-, Δ(galM-ybhJ)884, Δ46, met-100, [mal+]K-12(λS)
  WA837	5	F-, Δ(galM-ybhJ)884, Δ46, met-100, [mal+]K-12(λS), hsdR11
  WA834	6	F-, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, Δ46, met-100, [mal+]K-12(λS), hsdS10
  BL21(DE3)	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10
  BL21(DE3)/pCP20	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pCP20
  BL21(DE3)/pKD46	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pKD46
  BL21(DE3)/pLysS	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal+]K-12(λS), hsdS10, pLysS