CGSC Strain#: 6168
Strain Designation: NK5507      Source of Strain: N. Kleckner
Sex: F' Episome/Plasmid: F128-11
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), lacZp-4008(L8), lacI4500::Tn10
Chromosomal Markers: Δ(gpt-lac)5, thyA0, rpsL999(strR)
Strain Comments:

• lacIp4000 is called lacI^Q by B. Muller-Hill. lacZp4008 is called lacL8 by J. Beckwith et al.
• The derivation of the host strain has not been traced. J.Miller F-prime and background strain?
• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• thyA0-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• strR = streptomycin resist.
• = tetracycline resistant

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