CGSC Strain#: 6149
Strain Designation: HS4710      Source of Strain: H. Shuman
Sex: F' Episome/Plasmid: F110-10
   Plasmid Markers/Mutations: malG67
Chromosomal Markers: Δ(gpt-lac)5, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rpoB324(rifR), thiE1, metA28, malG67, deoC1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• thiE1 was formerly called thi-1
• fruA25 = former designation
• rifR = rifamycin resistance
• strR = streptomycin resist.

References:

• Silhavy, TJ, E Brickman, PJ Bassford, MJ Casadaban, HA Shuman, V Schwartz, L Guarente, M Schwartz, JR Beckwith 1979. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol. Gen. Genet. 174(3):249-59.