CGSC Strain#: 8294
Strain Designation: JW3164-1      Source of Strain: Keio Collection
Other Designations: JW3164
Sex: F- Chromosomal Markers: Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔkdsD726::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• From the Keio Collection of single-gene knockouts (See Baba et al. ref. below) jointly developed by the Keio Univ. Inst. of Adv. Biosci. and the Nara Inst. of Sci. and Technol., Japan, and Purdue Univ., USA.
• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
• ΔkdsD726::kan-- The primers used to make this knockout were GTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGATTCCGGGGATCCGTCGACC and TTTGCTCATTGTTGTTTATCCTTGAATCTTTACACTACGCCTGCACGCAGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
• ΔlacZ4787(::rrnB-3) was formerly called Δ lacZ_WJ16
• = kanamycin resistant
• ::rrnB-3 = 4 copies of rrnB inserted

References:

• Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11