CGSC Strain#: 8291
Strain Designation: BW11334/pBW120      Source of Strain: B.L. Wanner
Sex: F' Episome/Plasmid: F128-Tn10-11
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), ΔlacZ58(M15), F::Tn10-11, proA , proB , Tn10-11 , lac
Episome/Plasmid: pBW120
   Plasmid Markers/Mutations: bla(ApR), phnP-C
   Plasmid Comment: a pUC18 plasmid with insertion of the phn operon, plus the orfs originally designated phnA and phnB, a truncated orf phnQ, and an additional orf. Used for constructing several pWM plasmids.
Chromosomal Markers: Δ(argF-lac)169, recA1907::cat-aadA, thiE1, Δ(phnP-phnD)3330( phnC?), creC510
Strain Comments:

• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• recA1907::cat-aadA-- The aadA gene is expressed poorly and may confer low level Spectinomycin and streptomycin resistance
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• thiE1 was formerly called thi-1
• = chloramphenicol resistant
• = strep-resistant
• Const = constitutive
• phnC? = may also delete phnC
• = tetracycline resistant
• M15 = alpha-complemented allele
• ApR = ampicillin resistant

References:

• Wanner, B.L., J.A. Boline 1990. Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J.Bacteriol. 172:1186-1196