CGSC Strain#: 6097
Strain Designation: BW280      Source of Strain: B. Weiss
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, zfh-208::Tn10, nadB7, ung-1, relA1, spoT1, thiE1
Strain Comments:

• zfh-208::Tn10-- : insertion at nucleotide 2,665,159 (min. 57.448) within orf b2536 (yfhS208::Tn10)-Nichols et al. 1998.
• zfh-208::Tn10-- Cotransduction frequencies of zfh-208::Tn 10 with nearby markers: glyA 90%, purI 54%, nadB 8%, ung 4%.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• = tetracycline resistant

References:

• Duncan, BK, B Weiss 1982. Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli. J. Bacteriol. 151(2):750-5.
• Duncan, BK, B Weiss 1982. Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli. J. Bacteriol. 151(2):750-5.