CGSC Strain#: 5107
Strain Designation: CS101      Source of Strain: P.D. Skaar
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ompF627(T2R), fadL701(T2R), relA1, spoT1, metB1, rrnB-2, mcrB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Skaar, P.D., A. Garen 1956. The orientation and extent of gene transfer in Escherichia coli. Proc.Natl.Acad.Sci.USA 42:619-624