CGSC Strain#: 5988
Strain Designation: ST222      Source of Strain: H. Matsuzawa
Other Designations: H878
Other Designations: PC 1357
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), murC3(ts), secA216, fhuA61, lacY1, galT23, λ^-, trp-84, his-215, thyA710, rpsL263(strR), xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galT23-- : This is the standard gal₆ of Morse & Lederberg.
• thyA710-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• secA216 was formerly called azi-16
• secA216 was formerly called secA16
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Matsuzawa, H, M Matsuhashi, A Oka, Y Sugino 1969. Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochem. Biophys. Res. Commun. 36(4):682-9.
• Miyakawa, T., H. Matsuzawa, M. Matsuhashi, Y. Sugino 1972. Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes mra and mrb, for cell wall peptidoglycan biosynthesis. J.Bacteriol. 112:950-958