CGSC Strain#: 6368
Strain Designation: RL88      Source of Strain: R.E. Loughlin
Sex: F- Chromosomal Markers: galK2(Oc), λ^-, Δ(tonB-cysB)206, his-68, gyrA223(NalR), tyrA2, rpsL125(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• gyrA223 compensates for the deletion of topA in DE206. (R.E.Depew, personal communication)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• NalR = Nalidix.acidResist.
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Fimmel, AL, RE Loughlin 1977. Isolation of a lambdadcys transducing bacteriophage and its use in determining the regulation of cysteine messenger ribonucleic acid synthesis in Escherichia coli K-12. J. Bacteriol. 132(3):757-63.