CGSC Strain#: 6208
Strain Designation: KL651      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: serR-??-35, fhuA22, phoA4(Am), leuS31(ts), glnX44(AS), serS15(ts), hisG4(Oc), relA1, rpsL35(strR), leuR58, xylA5, mtl-1, spoT1, thiE1, T2R
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• leuS31(ts)-- is a temperature-sensitive mutation.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• serS15(ts)-- is a temperature-sensitive mutation.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• T2R-- : selected for resistance to phage T2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Theall, G., K.B. Low, D. Soll 1979. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase. Mol.Gen.Genet. 169:205-211