CGSC Strain#: 6513
Strain Designation: NM519      Source of Strain: N.E. Murray
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, sbcA23(Rac), his-60, relA1, recB21, recC22, rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, ΔhsdR24
Strain Comments:

• This strain was made by introducing hsdR24 into strain JC8679 of A.J.Clark.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• ΔhsdR24-- is an intragenic deletion.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Rac = within defective prophage Rac
• strR = streptomycin resist.

References:

• Sain, B., N.E. Murray 1980. The hsd (host specificity) genes of E. coli K12. Mol.Gen.Genet. 180:35-46