CGSC Strain#: 4117
Strain Designation: AB4117      Source of Strain: E.A. Adelberg
Sex: F' Episome/Plasmid: F8
   Plasmid Markers/Mutations:
Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), galK2(Oc), alaS5(ts), metC56, xyl-7 or xylA5, thiE1, tfr-5
Strain Comments:

• alaS5 is a temperature-sensitive mutation.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• ts = Temperature Sensitive

References:

• Theall, G, KB Low, D Soll 1977. Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity. Mol. Gen. Genet. 156(2):221-7.