CGSC Strain#: 4224
Strain Designation: KL141      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: λ^-, relA1, thyA25, argG6, gltB17, rpsL8 or rpsL104 or rpsL17, malT1(λ^R), pyrE41, rbs-1, thiE1
Strain Comments:

• Metzer reference below is for gltB17
• thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• gltB17 was formerly called gad-2
• gltB17 was formerly called gad-1
• thiE1 was formerly called thi-1
• = strep-resistant
• LamR = Lambda-resistant

References:

• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812
• Metzer, E., R. Levitz, Y.S. Halpern 1979. Isolation and properties of Escherichia coli K-12 mutants impaired in the utilization of gamma-aminobutyrate. J.Bacteriol. 137:1111-1118