CGSC Strain#: 4217
Strain Designation: KL253      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: galK35, λ^-, pyrD34, e14-, trpC45, his-68, tyrA2, recA1, rpsL118(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain has been tested for suppressors and none were found.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• strR = streptomycin resist.

References: