CGSC Strain#: 2295
Strain Designation: JC500      Source of Strain: A.J. Clark
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ^-, hns-1, hisG1, rfbC1, thyA3, galP63, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1, deoC5
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• thyA3-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• hns-1 was formerly called osmZ1
• hns-1 was formerly called cur-1
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Glassberg, J., R.R. Meyer, A. Kornberg 1979. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J.Bacteriol. 140:14-19