CGSC Strain#: 6124
Strain Designation: RH1-2      Source of Strain: R.L. Hanson
Sex: F- Chromosomal Markers: lacY1, tsx-29, glnX44(AS), galK2(Oc), λ^-, pnt-1, aroD6, rpsL700(strR), mtl-1, pgi-2
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• pgi-2-- : pgi mutants utilize glucose by the hexose monoP shunt; strains with this allele grew on glucose at 1/3 parental rate. Slow growth on Gal,Mal,Lac;normal on Fru and gluconate.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Hanson, RL, C Rose 1979. Genetic mapping of a mutation affecting pyridine nucleotide transhydrogenase in Escherichia coli. J. Bacteriol. 138(3):783-7.