CGSC Strain#: 6110
Strain Designation: KH54      Source of Strain: Horiuchi & Nagata
Sex: F- Chromosomal Markers: thr-33, λ^-, trpE9829(Am), serU126(ts,AS), his-2130, tyrA15(Am), thyA707, IN(rrnD-rrnE)1, ilv-683, metE46
Strain Comments:

• thyA707-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• serU126(ts,AS) was formerly called supD126
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• ts = Temperature Sensitive

References:

• Horiuchi, T, T Nagata 1973. Mutations affecting growth of the Escherichia coli cell under a condition of DNA polymerase I-deficiency. Mol. Gen. Genet. 123(1):89-110.