CGSC Strain#: 6108
Strain Designation: HD165      Source of Strain: M. Imai
Sex: F- Chromosomal Markers: thr-33, λ^-, tyrT666(ts,AS), trpE9829(Am), tyrA15(Am), thyA707, IN(rrnD-rrnE)1, ilv-683, rho-112(Am), argH212
Strain Comments:

• Note to ref. below: (describes closely related strains.)
• thyA707-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• ts = Temperature Sensitive

References:

• Imai, M, K Shigesada 1978. Studies on the altered rho factor in a nitA mutants of Escherichia coli defective in transcription termination. I. Characterization and quantitative determination of rho in cell extracts. J. Mol. Biol. 120(4):451-66.