CGSC Strain#: 6106
Strain Designation: HD152      Source of Strain: M. Imai
Sex: F- Chromosomal Markers: thr-33, λ^-, trpE9829(Am), serU126(ts,AS), his-2130, tyrA15(Am), thyA707, IN(rrnD-rrnE)1, rho-702(ts)
Strain Comments:

• Note that trpE9829 and tyrA15 are amber mutations. rho-702 and serU126 (amber suppressor) are temperature-sensitive mutations.
• thyA707-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• serU126(ts,AS) was formerly called supD126
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• ts = Temperature Sensitive

References:

• Inoko, H., K. Shigesada, M. Imai 1977. Isolation and characterization of conditional-lethal rho mutants of Escherichia coli. Proc.Natl.Acad.Sci.USA 74:1162-1166
• Imai, M, K Shigesada 1978. Studies on the altered rho factor in a nitA mutants of Escherichia coli defective in transcription termination. I. Characterization and quantitative determination of rho in cell extracts. J. Mol. Biol. 120(4):451-66.