CGSC Strain#: 5854
Strain Designation: CS425      Source of Strain: C.R. Somerville
Sex: F- Chromosomal Markers: araC14, leuB6(Am), lacZ36, proC32, λ^-, hisF860(Stable), cysJ43, thyA54, rpsE2015(SpcR), rpsL109(strR), xylA5, mtl-1, gpp-2, thiE1
Strain Comments:

• This strain may carry a malA mutation ( malA38) and a suppressor near his ( sup-78) which confers a Mal⁺ phenotype.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• cysJ43-- - the cys allele was formerly thought to be in cysC.
• thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• proC32 was formerly called proC65
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• SpcR = spectinomycin resistant
• Stable = stable, non-reverting
• strR = streptomycin resist.

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