CGSC Strain#: 5852
Strain Designation: CS416      Source of Strain: C.R. Somerville
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, hisF860(Stable), cysJ43, thyA54, rpsE2015(SpcR), rpsL109(strR), xyl-7 or xylA5, gpp-1, metE70, thiE1
Strain Comments:

• This strain could carry the mutation malA38, which confers LAMR, and a suppressor, sup-78, conferring a Mal⁺, LAM^S phenotype.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• cysJ43-- - the cys allele was formerly thought to be in cysC.
• thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• SpcR = spectinomycin resistant
• Stable = stable, non-reverting
• strR = streptomycin resist.

References:

• Somerville, C.R., A. Ahmed 1979. Mutants of Escherichia coli defective in the degradation of guanosine 5'-triphosphate, 3'-diphosphate (pppGpp). Mol.Gen.Genet. 169:315-323