CGSC Strain#: 8255
Strain Designation: NCM533      Source of Strain: N. Majdalani
Sex: F- Chromosomal Markers: Δ(araD-araB)714, Δ(lacA-lacZ)880(::FRT), lacIp-4000(lacI^Q), zah-2225::FRT, λ^-, ΔaraEp-531::kan, φP_cp18araE533, rph-1
Strain Comments:

• Δ(lacA-lacZ)880(::FRT)-- is the DE(lacA-lacZ)879::kan deletion with the kan cassette removed
• zah-2225::FRT-- is the same as zah-2224::cat with the cat cassette removed
• ΔaraEp-531::kan-- has resistance marker useful for transfer of F-prime carrying Pcp- araE fusion.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant
• ::FRT = Flp Recombinase Target

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