CGSC Strain#: 8251
Strain Designation: NCM517      Source of Strain: N. Majdalani
Sex: F- Chromosomal Markers: Δ(lacA-lacZ)879(::kan), λ^-, rph-1
Strain Comments:

• Δ(lacA-lacZ)879(::kan)-- extends from the -35 of lacZ through the stop codon of lacA. The kan cassette is flanked by FRT sites
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

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