CGSC Strain#: 8249
Strain Designation: NCM514      Source of Strain: N. Majdalani
Sex: F- Chromosomal Markers: lacIp-4000(lacI^Q), zah-2224::cat, λ^-, rph-1
Strain Comments:

• zah-2224::cat-- The cat cassette is located 2 bases after the stop codon of mhpR and 11 bases upstream of the C to T mutation in the -35 of the lacI promoter, it is flanked by FRT sites.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = chloramphenicol resistant

References: