CGSC Strain#: 7804
Strain Designation: K1640      Source of Strain: Russel & Model
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, phoA4(Am), ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, Δ(atpC-atpI)750, ΔtrxA307, rrnB-2, mcrB1, creC510
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(atpC-atpI)750-- Deleted region includes at least atpCDGAHFEBI
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Am = amber(UAG) mutation
• Const = constitutive
• T2R = T2 Resistant

References:

• Feng, J., M. Russel, P. Model 1997. A permeabilized cell system that assembles filamentous bacteriophage. Proc.Natl.Acad.Sci.USA 94:4068-4073