CGSC Strain#: 8118
Strain Designation: BGS403      Source of Strain: E.J. Murgola
Sex: F' Episome/Plasmid: F596-12
   Plasmid Markers/Mutations: colI+, colV+, colB+, trpA213(UGA,UGA211)
   Plasmid Comment: This episome contains genes from Col plasmids for colicins V and B and the tonB-cysB region of the chromosome, which includes the trp operon as well as the suppressible trpA mutation.
Chromosomal Markers: λ^-, Δ(tonB-trp), IN(rrnD-rrnE)1, rph-1, glyV55
Strain Comments:

• The episome is a derivative of the Fredericq F-prime and contains genes for colicins V and B and the tonB-cysB region of the chromosome that includes the trp operon, with a suppressible trpA mutation.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• glyV55-- reads GGA/G
• UGA = opal(UGA) mutation
• UGA211 = basepr. mut.of trpA211(UGA211)

References:

• Arkov, A.L., A. Mankin, E.J. Murgola 1998. An rRNA fragment and its antisense can alter decoding of genetic information. J.Bacteriol. 180:2744-2748
• Chernyaeva, N.S., E.J. Murgola, A.S. Mankin 1999. Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA. J.Bacteriol. 181:5257-5262