CGSC Strain#: 5872
Strain Designation: ZIP195F      Source of Strain: D. Zipser
Sex: F' Episome/Plasmid: F42-521
   Plasmid Markers/Mutations: lacZ521(UGA), lac
Chromosomal Markers: lacZ521(UGA), λ^-, trpA49(Am), relA1, spoT1
Strain Comments:

• N.B. This strain is a homogenote for the UGA mutation lacZ521.
• lacZ521(UGA)-- is an UGA mutation in region 13 of the lacZ gene, as described by Zipser et al. 1970.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Am = amber(UAG) mutation
• UGA = opal(UGA) mutation
• UGA = opal(UGA) mutation

References:

• Zipser, D. 1967. J.Mol.Biol. 29:441-0
• Zipser, D, S Zabell, J Rothman, T Grodzicker, M Wenk 1970. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49(1):251-4.