CGSC Strain#: 5424
Strain Designation: BB20-14      Source of Strain: Cronan & Bell
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR2(glp^c), glpD3, pitA10, gpsA20, spoT1, glpK15(fbR), rrnB-2, mcrB1, creC510
Strain Comments:

• glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
• glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glpK15(fbR)-- kinase insensitive to feedback inhibition
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• fbR = feedback resistant
• glp^c = constitutive expr.glp regulon
• T2R = T2 Resistant

References:

• Bell, RM 1974. Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J. Bacteriol. 117(3):1065-76.
• Cronan, J.E., R.M. Bell 1974. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J.Bacteriol. 118:598-605