CGSC Strain#: 8185
Strain Designation: BW31005      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, att80(CmR)::LZ44-lacZp-GFP, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• Insertion of pLZ44 at att_φ80 site, resulting in GFP on the chromosome, inducible by IPTG. Also CmR from pLZ44 is present.
• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
• ΔlacZ4787(::rrnB-3) was formerly called Δ lacZ_WJ16
• ::rrnB-3 = 4 copies of rrnB inserted
• CmR = chloramphenicol resistant

References:

• Zhou, L., K. Zhang, B.L. Wanner 2004. Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli. Methods Mol Biol 267:123-134